Puey Ounjai Lab

Department of Biology, Faculty of Science, Mahidol University

Publication

Journal Covers:

2023 – ScienceAsia (Feb 2023)

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2017- Essays in Biochemistry (Mar 2017)

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2013- Current Biology (Feb 2013); AIP Rev Sci Inst (Nov 2013)

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2012- Biol Reprod (Sep 2012)

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Preprints:

Sonpho E, Mann Jr. FG, Levy M,  Ross EJGuerrero-Hernández CFlorens L,  Saraf A, Doddihal V, Ounjai PSánchez Alvarado A. (2020) Decellularization enables functional analysis of ECM remodelling in planarian regeneration.
https://www.biorxiv.org/content/10.1101/2020.09.11.293936v2 

Sonpho E, Wootthichairangsan C, Ishida M, Inoue T, Agata K, Maleehuan A, Charngkaew K, Chomanee N, Moonsom S, Wongtrakoongate P, Chairoungdua A, Ounjai P. (2019) ECM-Body: A Cell-Free 3D Biomimetic Scaffold Derived from Intact Planarian Body. 
https://www.biorxiv.org/content/10.1101/763714v1


Peer-Reviewed Articles:

2023 –

Manan A, Roytrakul S, Charoenlappanit S, Poolpak T, Ounjai P, Kruatrachue M, Yang KM, Pokethitiyook P* (2023) Glyphosate metabolism in Tetrahymena thermophila: A shotgun proteomic analysis approach. Environmental Toxicology, doi: 10.1002/tox.23735. 

Chusuth P, Raksat P, Hannongbua S, Wangkanont K*, Ounjai P*, Rungrotmongkol T* (2023) Expression and Purification of human alpha-7 nicotinic acetylcholine receptor extracellular domain in Pichia pastoris. ScienceAsia. 49: 8-14 doi: 10.2306/scienceasia1513-1874.2022.110

Wannigama DL*, Amarasiri M, Hongsing P, Hurst C, Modchang C, Chadsuthi S, Anupong S, Phattharapornjaroen P, S M AHR, Fernandez S, Huang AT, Kueakulpattana N, Tanasatitchai C, Vatanaprasan P, Saethang T, Luk-In S, Storer RJ, Ounjai P, Ragupathi NKD, Kanthawee P, Sano D, Furukawa T, Sei K, Leelahavanichkul A, Kanjanabuch T, Hirankarn N, Higgins PG, Kicic A, Chatsuwan T, McLellan AD, Abe S. (2023)  Multiple traces of monkeypox detected in non-sewered wastewater with sparse sampling from a densely populated metropolitan area in Asia. Science of Total Environment. 858(Pt 1):159816. doi: 10.1016/j.scitotenv.2022.159816. 

2022-

Weerachatyanukul W*, Kiatmetha P, Raksat P, Boonkua S, Thongsum O, Jariyapong P, Chotwiwatthanakun C, Ounjai P, Metlagel Z (2022) Viral Capsid Change upon Encapsulation of Double-Stranded DNA into an Infectious Hypodermal and Hematopoietic Necrosis Virus-like Particle. Viruses. 15(1):110. doi: 10.3390/v15010110. 

Shein AMS, Wannigama DL*, Higgins PG, Hurst C, Abe S, Hongsing P, Chantaravisoot N, Saethang T, Luk-in S, Liao T, Nilgate S, Rirerm U, Kueakulpattana N, Srisakul S, Aryukarn A, Laowansiri M, Hao LY, Yonpiam M, Ragupathi NKD, Techawiwattanaboon, T, Ngamwongsathit N, Amarasiri M, Ounjai P, Kupwiwat R, Phattharapornjaroen P, Badavath VN, Leelahavanichkul A, Kicic A, Chatsuwan T* (2022) High prevalence of mgrB-mediated colistin resistance among carbapenem-resistant Klebsiella pneumoniae is associated with biofilm formation, and can be overcome by colistin-EDTA combination therapy. Scientific Reports. 12 (1):12939. doi: 10.1038/s41598-022-17083-5.

Kespichayawattana N, Jantarat J*, Ounjai P, Wongtrakoongate P. (2022) Cytotoxic effect of gutta percha solvents on human periodontal ligament fibroblasts. Mahidol Dental Journal. 42(2):137-144.

2021 –

Sonpho E, Mann FG Jr, Levy M, Ross EJ, Guerrero-Hernández C, Florens L, Saraf A, Doddihal V, Ounjai P, Sánchez Alvarado A* (2021) Decellularization Enables Characterization and Functional Analysis of Extracellular Matrix in Planarian Regeneration. Molecular & Cellular Proteomics. 20:100137. doi: 10.1016/j.mcpro.2021.100137.

Ojha SC, Phanchana M, Harnvoravongchai P, Chankhamhaengdecha S, Singhakaew S, Ounjai P, Janvilisri T* (2021) Teicoplanin Suppresses Vegetative Clostridioides difficile and Spore Outgrowth. Antibiotics (Basel). 10(8):984. doi: 10.3390/antibiotics10080984.

Asawasriworanan T, Harnvoravongchai P, Somana J, Chankhamhaengdecha S*, Ounjai P* (2021) Draft Genome Sequence of Neobacillus cucumis Strain T4S4, a Stevioside and Rebaudioside A Hydrolytic Strain Isolated from Tropical Forest Soil. Microbiol Resour Announc, 10(21):e0149120. doi: 10.1128/MRA.01491-20.

Wannigama DL, Amarasiri M, Hurst C, Phattharapornjaroen P, Abe S, Hongsing P, Hosseini Rad SMA, Pearson L, Saethang T, Luk-in S, Kueakulpattana N, Storer RJ, Ounjai P, Kavquet A, Leelahavanichkul A, Chatsuwan T* (2021) Tracking COVID-19 with wastewater to understand asymptomatic transmission. Int J Infect Dis, S1201-9712(21)00406-9. doi: 10.1016/j.ijid.2021.05.005.

Promsing S, Pokethitiyook P*, Kruatrachue M, Ounjai P, Meetam M, Onparn N, Kumsopa A (2021) Rhizoremediation of fuel oil by Vetiveria zizanioides in association with Kocuria no MU1 and Micrococcus luteus WN01. ScienceAsia, 47; 95-105. Doi: 10.2306/scienceasia1513-1874.2021.015.

Sridapan T , Tangkawsakul W, Janvilisri T, Kiatpathomchai W, Dangtip S, Ngamwongsatit N, Nacapricha D, Ounjai P, Chankhamhaengdecha S. (2021) Rapid detection of Clostridium perfringens in food by loop-mediated isothermal amplification combined with a lateral flow biosensor. PLoS One, 16(1):e0245144. doi: 10.1371/journal.pone.0245144. 

2020 – 
Sonpho E
, Wootthichairangsan C, Ishida M, Inoue T, Agata K, Maleehuan A, Charngkaew K, Chomanee N, Moonsom S, Wongtrakoongate P, Chairoungdua A, Ounjai P. (2020) ECM-Body: A Cell-Free 3D Biomimetic Scaffold Derived from Intact Planarian Body. Zoological Science, 37(4):307-313.doi: 10.2108/zs190135.

Harnvoravongchai P, Singwisut R, Ounjai P, Aroonnual A, Kosiyachinda P, Janvilisri T, Chankhamhaengdecha S. (2020) Isolation and characterization of thermophilic cellulose and hemicellulose degrading bacterium, Thermoanaerobacterium sp. R63 from tropical dry deciduous forest soil. PLOS ONE, 15(7):e0236518.doi: 10.1371/journal.pone.0236518.

Kitdumrongthum S, Reabroi S, Suksen K, Tuchinda P, Munyoo B, Mahalapbutr P, Rungrotmongkol T, Ounjai P, Chairoungdua A. (2020) Inhibition of topoisomerase IIα and induction of DNA damage in cholangiocarcinoma cells by altholactone and its halogenated benzoate derivatives. Biomedicine and Pharmacotherapy, 127:110149.doi: 10.1016/j.biopha.2020.110149.

Phanchana M, Phetruen T, Harnvoravongchai P, Raksat P, Ounjai P, Chankhamhaengdecha S, Janvilisri T* (2020) Repurposing a platelet aggregation inhibitor ticagrelor as an antimicrobial against Clostridioides difficile. Scientific Reports. 10:6497. doi: 10.1038/s41598-020-63199-x.

Jampasri K, Pokethitiyook P*, Poolpak T, Kruatrachue M, Ounjai P, Kumsopa A (2020) Bacteria-assisted phytoremediation of fuel oil and lead co-contaminated soil in the salt-stressed condition by Chromolaena odorata and Micrococcus luteus. International Journal of  Phytoremediation, 1-12. doi: 10.1080/15226514.2019.1663482

2019 –
Samranwanich T, Boonthaworn K, Singhakaew S*, Ounjai P* (2019) Time-Restricted Inquiry-Based Learning Promotes Student Active Engagement in Undergraduate Zoology Laboratory. Journal of Microbiology and Biology Education. pii: 20.1.2. doi: 10.1128/jmbe.v20i1.1571 

Kampeera J, Pasakon P, Karuwan C, Arunrut N, Sappat A, Sirithammajak S, Dechokiattawan N, Sumranwanich T, Chaivisuthangkura P, Ounjai P, Chankhamhaengdecha S, Wisitsoraat A, Tuantranont A, Kiatpathomchai W* (2019) Point-of-care rapid detection of Vibrio parahaemolyticus in seafood using loop-mediated isothermal amplification and graphene-based screen-printed electrochemical sensor. Biosensors and Bioelectronics, 132:271-278. doi:10.1016/j.bios.2019.02.060.

Taweewattanapaisan P, Jantarat J*, Ounjai P, Janebodin K. (2019) The Effects of EDTA on Blood Clot in Regenerative Endodontic Procedures. Journal of Endodontics, 45(3):281-286. doi: 10.1016/j.joen. 2018.10.010.

2018 –
Kiatmetha P
, Chotwiwatthanakun C, Jariyapong P, Santimanawong W, Ounjai P, Weerachatyanukul W* (2018) Nanocontainer designed from an infectious hypodermal and hematopoietic necrosis virus (IHHNV) has excellent physical stability and ability to deliver shrimp tissues. Peer Journal,  doi: 10.7717/peerj.6079.

Subsoontorn P*, Ounjai P, Ngarmkajornwiwat P, Sakulkueakulsuk B, Pensupha N, Surareungchai W, Pataranutaporn P (2018) Hack Biodesign: An Integrative STEAM Education Platform for Biology, Engineering, and Design. IEEE TALE, DOI: 10.1109/TALE.2018.8615404.

Harnvoravongchai P, Chankhamhaengdecha S, Ounjai P, Singhakaew S, Boonthaworn K, Janvilisri T* (2018) Antimicrobial Effect of Asiatic Acid Against Clostridium difficile Is Associated With Disruption of Membrane Permeability. Frontiers in Microbiology, https://doi.org/ 10.3389/ fmicb.2018.02125

Kitdumrongthum S, Metheetrairut C, Charoensawan V, Ounjai P, Janpipatkul K, Panvongsa W, Weerachayaphorn J, Piyachaturawat P, Chairoungdua A* (2018) Dysregulated microRNA expression profiles in cholangiocarcinoma cell-derived exosomes. Life Science pii: S0024-3205(18)30520-4. doi: 10.1016/j.lfs.2018.08.058

Phothichaisri W, Ounjai P, Phetruen T, Janvilisri T, Khunrae P, Singhakaew S, Wangroongsarb P, Chankhamhaengdecha S* (2018) Characterization of Bacteriophages Infecting Clinical Isolates of Clostridium difficile, Frontiers in Microbiology, https://doi.org/10.3389/fmicb.2018. 01701

Hanpaibool C, Chakcharoensap T, Arifin, Hijikata Y, Irle S, Wolschann P,  Kungwan N, Pongsawasdi P, Ounjai P*, Rungrotmongkol T* (2018) Theoretical analysis of orientations and tautomerization of genistein in β-cyclodextrin. Journal of Molecular Liquids. 265: 16-23. doi: 10.1016/j.molliq.2018.05.109.

Reynolds MJ, Phetruen T, Fisher RL, Chen K, Pentecost BT, Gomez G, Ounjai P, Sui H* (2018) The Developmental Process of the Growing Motile Ciliary Tip Region. Scientific Reports, 8(1):7977. doi: 10.1038/s41598-018-26111-2.

2017 –
Ramphan S, Suksathan S, Wikan N, Ounjai P, Boonthaworn K, Rimthong P, Kanjanapruthipong T, Worawichawong S, Jongkaewwattana A, Wongsiriroj N, Smith DR* (2017) Oleic acid Enhances Dengue Virus but Not Dengue Virus-Like Particle Production from Mammalian Cells, Mol Biotechnol. doi: 10.1007/s12033-017-0029-4.  

Aroonnual A, Janvilisri T, Ounjai P, Chankhamhaengdecha S* (2017) Microfluidics: Innovative Approaches for Rapid Diagnosis of Antibiotic Resistant Bacteria, Essays in Biochem. 61(1):91-101. doi: 10.1042/EBC20160059 (Featured as cover)

Somrit M, Watthammawut A, Chotwiwatthanakun C, Ounjai P, Suntimanawong W, Weerachatyanukul W*, (2017) C-terminal domain on the outer surface of the Macrobrachium rosenbergii Nodavirus capsid is required for Sf9 cell binding and internalization. Virus Res. 2016 Sep 28;227:41-48. doi: 10.1016/j.virusres.2016.09.017.

2016 –
Jampasri K, Pokethitiyook P*, Kruatrachue M, Ounjai P, Kumsopa A, (2016) Phytoremediation of fuel oil and lead co-contaminated soil by Chromolaena odorata in association with Micrococcus luteus. Int J Phytoremediation. 2016 Oct 2;18(10):994-1001.

Singhakaew S, Boonthaworn K, Sonpho E, Ounjai P*, (2016) An Overview of Sample Preparation Methods for Biological Electron Microscopy. Siriraj Med J 2016;68:(Supl 1):S91-S95

Charngkaew K, Boonthaworn K, Sonpho E, Chomanee N, Ounjai P*, (2016) A Primer to Electron Tomography. Siriraj Med J 2016;68:(Supl 1):S86-S90.

Liu Y, Tran BN, Wang F, Ounjai P., Wu JL*, Hew CL*, (2016) Visualization of Assembly Intermediates and Budding Vacuoles of Singapore Grouper Iridovirus in Grouper Embryonic Cells, Scientific Reports, 6:18696. doi: 10.1038/srep18696.

Ojha SC, Chankhamhaengdecha S, Singhakaew S, Ounjai P, Janvilisri T*, (2016) Inactivation of Clostridium difficile spores by microwave irradiation. Anaerobe, S1075-9964(15)30075-5. doi: 10.1016/j.anaerobe.2015.10.015.

2014 –
Chaturongakul S, Ounjai P*, (2014) Phage-host interplay: examples from tailed phages and Gram-negative bacterial pathogens. Frontiers in Microbiology, 5:442. doi:10.3389/fmicb, 2014.00442. 

2013 –
Ounjai P, Kim KD, Liu H, Dong M, Tauscher AN, Witkowska HE, Downing KH* (2013) Architectural insights into a ciliary partition. Current Biology, 23: 339-44 (Featured as cover), recommended by F1000. 

2012 –
Ounjai P
, Kim, KD, Lishko PV, Downing KH* (2012) Three Dimensional Structure of Bovine Sperm Connecting Piece Revealed by Electron Cryotomography., Biology of Reproduction, 87 (73), 1-9  (Featured as cover)

2008 –
Wang L, Ounjai P, Sigworth, FJ*  (2008) Streptavidin crystals as nanostructured supports and image-calibration references for cryo-EM data collection. Journal of Structural Biology, 164(2):190-8.

2007 –
Ounjai P, Unger VM, Sigworth FJ, Angsuthanasombat C* (2007) Two conformational states of the membrane-associated Bacillus thuringiensis Cry4Ba δ-endotoxin complex revealed by electron crystallography: Implications for toxin-pore formation. Biochemical Biophysical Research Communications 361(4):890-5. 

2004 –
Angsuthanasombat C, Uawithya P, Leetachewa S, Pornwiroon W, Ounjai P, Kerdchareon T, Katzenmeier G, Panyim S* (2004) Bacillus thuringiensis Cry4A and Cry4B mosquito-larvicidal proteins: Homology-based 3D model and implications for toxin activity. Journal of Biochemistry and Molecular Biology 37(3):304-313.

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